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Table 4 Microglial ultrastructure in both layers of the dorsal hippocampus CA1

From: Microglia are involved in phagocytosis and extracellular digestion during Zika virus encephalitis in young adult immunodeficient mice

Parameters

Number per cell (mean ± SEM)

Stratum radiatum

Stratum lacunosum-moleculare

Day 0

Day 7 p.i.

Day 0

Day 7 p.i.

 Organelles of phagolysosomal pathway within microglia

  Lysosomes

Immatures

0.031 ± 0.031

0.406 ± 0.126*

0.313 ± 0.123

0.306 ± 0.087

Matures

0.094 ± 0.052

0.250 ± 0.078

0.344 ± 0.124

0.333 ± 0.089

Total

0.125 ± 0.059

0.656 ± 0.159*

0.656 ± 0.159

0.639 ± 0.139

  Endosomes

Empty

0.094 ± 0.052

0.188 ± 0.105

0.063 ± 0.043

0.222 ± 0.081

With content

0.531 ± 0.180

0.281 ± 0.112

0.719 ± 0.175

0.472 ± 0.116

Total

0.625 ± 0.178

0.469 ± 0.201

0.781 ± 0.189

0.694 ± 0.178

  Lipidic inclusions

 

0.156 ± 0.079

0.250 ± 0.089

0.250 ± 0.100

0.417 ± 0.108

 Altered organelles within microglia

  ER/Golgi

Dilation

0.875 ± 0.059

0.781 ± 0.074

0.906 ± 0.052

0.944 ± 0.039

  Mitochondria

Elongated

0.188 ± 0.095

0.438 ± 0.134

0.594 ± 0.173

0.417 ± 0.128

Holy

0.094 ± 0.052

0.094 ± 0.069

0.094 ± 0.052

0.139 ± 0.058

Donut-shaped

0.031 ± 0.031

0 ± 0

0.031 ± 0.031

0.055 ± 0.039

Abnormal

0.281 ± 0.103

0.375 ± 0.117

0.688 ± 0.182

0.611 ± 0.175

Total (normal + altered)

2.188 ± 0.319

3.531 ± 0.741

3.531 ± 0.552

3.833 ± 0.531

 Contacts of microglia with microenvironment

  Synaptic elements

  (neurons)

Pre-synaptic

4.406 ± 0.638

3.375 ± 0.451

5.813 ± 0.595

4.944 ± 0.569

Post-synaptic

1.875 ± 0.361

2.094 ± 0.352

3.219 ± 0.380

2.389 ± 0.439*

  Myelin

  (neurons)

Myelinated axons

0.344 ± 0.115

0.313 ± 0.138

0.625 ± 0.290

0.333 ± 0.098

Degraded myelin

0.469 ± 0.149

0.969 ± 0.260

1.250 ± 0.220

0.778 ± 0.200 ( P = 0.05)

  Brain cells or vasculature

Neuronal cell bodies

0.189 ± 0.095

0.063 ± 0.043

0.031 ± 0.031

0.083 ± 0.047

Astrocytic cell bodies

0.031 ± 0.031

0.031 ± 0.031

0.219 ± 0.087

0.306 ± 0.078

Blood vessels

0.156 ± 0.065

0.094 ± 0.052

0.156 ± 0.079

0.139 ± 0.058

  Myelin debris

 

0.500 ± 0.135

0.594 ± 0.184

0.906 ± 0.217

0.722 ± 0.181

  Extracellular space

 

3.935 ± 0.510

2.625 ± 0.399

3.531 ± 0.557

2.750 ± 0.517

  Extracellular digestion

 

0.438 ± 0.174

1.194 ± 0.199**

0.281 ± 0.121

0.639 ± 0.121*

  1. ER/Golgi, endoplasmic reticulum and Golgi apparatus cisterna; p.i., post-infection; SEM, standard error of the mean
  2. Results are the mean ± SEM of 32–33 microglia per time point (6–10 microglia per animal for a total of 4 mice per time point) in the stratum radiatum and of 32–36 microglia per time point (7–10 microglia per animal for a total of 4 mice per time point) in the stratum lacunosum-moleculare
  3. Statistical analyses were performed using a nonparametric Mann-Whitney comparison test. Results that are statistically different compared to day 0 (noninfected mice) are presented as follows: * P < 0.05; ** P < 0.01 and are indicated in bold