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Table 4 Microglial ultrastructure in both layers of the dorsal hippocampus CA1

From: Microglia are involved in phagocytosis and extracellular digestion during Zika virus encephalitis in young adult immunodeficient mice

Parameters Number per cell (mean ± SEM)
Stratum radiatum Stratum lacunosum-moleculare
Day 0 Day 7 p.i. Day 0 Day 7 p.i.
Organelles of phagolysosomal pathway within microglia
  Lysosomes Immatures 0.031 ± 0.031 0.406 ± 0.126* 0.313 ± 0.123 0.306 ± 0.087
Matures 0.094 ± 0.052 0.250 ± 0.078 0.344 ± 0.124 0.333 ± 0.089
Total 0.125 ± 0.059 0.656 ± 0.159* 0.656 ± 0.159 0.639 ± 0.139
  Endosomes Empty 0.094 ± 0.052 0.188 ± 0.105 0.063 ± 0.043 0.222 ± 0.081
With content 0.531 ± 0.180 0.281 ± 0.112 0.719 ± 0.175 0.472 ± 0.116
Total 0.625 ± 0.178 0.469 ± 0.201 0.781 ± 0.189 0.694 ± 0.178
  Lipidic inclusions   0.156 ± 0.079 0.250 ± 0.089 0.250 ± 0.100 0.417 ± 0.108
Altered organelles within microglia
  ER/Golgi Dilation 0.875 ± 0.059 0.781 ± 0.074 0.906 ± 0.052 0.944 ± 0.039
  Mitochondria Elongated 0.188 ± 0.095 0.438 ± 0.134 0.594 ± 0.173 0.417 ± 0.128
Holy 0.094 ± 0.052 0.094 ± 0.069 0.094 ± 0.052 0.139 ± 0.058
Donut-shaped 0.031 ± 0.031 0 ± 0 0.031 ± 0.031 0.055 ± 0.039
Abnormal 0.281 ± 0.103 0.375 ± 0.117 0.688 ± 0.182 0.611 ± 0.175
Total (normal + altered) 2.188 ± 0.319 3.531 ± 0.741 3.531 ± 0.552 3.833 ± 0.531
Contacts of microglia with microenvironment
  Synaptic elements
  (neurons)
Pre-synaptic 4.406 ± 0.638 3.375 ± 0.451 5.813 ± 0.595 4.944 ± 0.569
Post-synaptic 1.875 ± 0.361 2.094 ± 0.352 3.219 ± 0.380 2.389 ± 0.439*
  Myelin
  (neurons)
Myelinated axons 0.344 ± 0.115 0.313 ± 0.138 0.625 ± 0.290 0.333 ± 0.098
Degraded myelin 0.469 ± 0.149 0.969 ± 0.260 1.250 ± 0.220 0.778 ± 0.200 ( P = 0.05)
  Brain cells or vasculature Neuronal cell bodies 0.189 ± 0.095 0.063 ± 0.043 0.031 ± 0.031 0.083 ± 0.047
Astrocytic cell bodies 0.031 ± 0.031 0.031 ± 0.031 0.219 ± 0.087 0.306 ± 0.078
Blood vessels 0.156 ± 0.065 0.094 ± 0.052 0.156 ± 0.079 0.139 ± 0.058
  Myelin debris   0.500 ± 0.135 0.594 ± 0.184 0.906 ± 0.217 0.722 ± 0.181
  Extracellular space   3.935 ± 0.510 2.625 ± 0.399 3.531 ± 0.557 2.750 ± 0.517
  Extracellular digestion   0.438 ± 0.174 1.194 ± 0.199** 0.281 ± 0.121 0.639 ± 0.121*
  1. ER/Golgi, endoplasmic reticulum and Golgi apparatus cisterna; p.i., post-infection; SEM, standard error of the mean
  2. Results are the mean ± SEM of 32–33 microglia per time point (6–10 microglia per animal for a total of 4 mice per time point) in the stratum radiatum and of 32–36 microglia per time point (7–10 microglia per animal for a total of 4 mice per time point) in the stratum lacunosum-moleculare
  3. Statistical analyses were performed using a nonparametric Mann-Whitney comparison test. Results that are statistically different compared to day 0 (noninfected mice) are presented as follows: * P < 0.05; ** P < 0.01 and are indicated in bold