Parameters | Number per cell (mean ± SEM) | ||||
---|---|---|---|---|---|
Stratum radiatum | Stratum lacunosum-moleculare | ||||
Day 0 | Day 7 p.i. | Day 0 | Day 7 p.i. | ||
 Organelles of phagolysosomal pathway within microglia | |||||
  Lysosomes | Immatures | 0.031 ± 0.031 | 0.406 ± 0.126* | 0.313 ± 0.123 | 0.306 ± 0.087 |
Matures | 0.094 ± 0.052 | 0.250 ± 0.078 | 0.344 ± 0.124 | 0.333 ± 0.089 | |
Total | 0.125 ± 0.059 | 0.656 ± 0.159* | 0.656 ± 0.159 | 0.639 ± 0.139 | |
  Endosomes | Empty | 0.094 ± 0.052 | 0.188 ± 0.105 | 0.063 ± 0.043 | 0.222 ± 0.081 |
With content | 0.531 ± 0.180 | 0.281 ± 0.112 | 0.719 ± 0.175 | 0.472 ± 0.116 | |
Total | 0.625 ± 0.178 | 0.469 ± 0.201 | 0.781 ± 0.189 | 0.694 ± 0.178 | |
  Lipidic inclusions |  | 0.156 ± 0.079 | 0.250 ± 0.089 | 0.250 ± 0.100 | 0.417 ± 0.108 |
 Altered organelles within microglia | |||||
  ER/Golgi | Dilation | 0.875 ± 0.059 | 0.781 ± 0.074 | 0.906 ± 0.052 | 0.944 ± 0.039 |
  Mitochondria | Elongated | 0.188 ± 0.095 | 0.438 ± 0.134 | 0.594 ± 0.173 | 0.417 ± 0.128 |
Holy | 0.094 ± 0.052 | 0.094 ± 0.069 | 0.094 ± 0.052 | 0.139 ± 0.058 | |
Donut-shaped | 0.031 ± 0.031 | 0 ± 0 | 0.031 ± 0.031 | 0.055 ± 0.039 | |
Abnormal | 0.281 ± 0.103 | 0.375 ± 0.117 | 0.688 ± 0.182 | 0.611 ± 0.175 | |
Total (normal + altered) | 2.188 ± 0.319 | 3.531 ± 0.741 | 3.531 ± 0.552 | 3.833 ± 0.531 | |
 Contacts of microglia with microenvironment | |||||
  Synaptic elements   (neurons) | Pre-synaptic | 4.406 ± 0.638 | 3.375 ± 0.451 | 5.813 ± 0.595 | 4.944 ± 0.569 |
Post-synaptic | 1.875 ± 0.361 | 2.094 ± 0.352 | 3.219 ± 0.380 | 2.389 ± 0.439* | |
  Myelin   (neurons) | Myelinated axons | 0.344 ± 0.115 | 0.313 ± 0.138 | 0.625 ± 0.290 | 0.333 ± 0.098 |
Degraded myelin | 0.469 ± 0.149 | 0.969 ± 0.260 | 1.250 ± 0.220 | 0.778 ± 0.200 ( P = 0.05) | |
  Brain cells or vasculature | Neuronal cell bodies | 0.189 ± 0.095 | 0.063 ± 0.043 | 0.031 ± 0.031 | 0.083 ± 0.047 |
Astrocytic cell bodies | 0.031 ± 0.031 | 0.031 ± 0.031 | 0.219 ± 0.087 | 0.306 ± 0.078 | |
Blood vessels | 0.156 ± 0.065 | 0.094 ± 0.052 | 0.156 ± 0.079 | 0.139 ± 0.058 | |
  Myelin debris |  | 0.500 ± 0.135 | 0.594 ± 0.184 | 0.906 ± 0.217 | 0.722 ± 0.181 |
  Extracellular space |  | 3.935 ± 0.510 | 2.625 ± 0.399 | 3.531 ± 0.557 | 2.750 ± 0.517 |
  Extracellular digestion |  | 0.438 ± 0.174 | 1.194 ± 0.199** | 0.281 ± 0.121 | 0.639 ± 0.121* |