Fig. 1

Complement inhibitors rescue alpha-synuclein-induced toxicity in SH-SY5Y cells. A Non-mitotic transgenic SH-SY5Y cells expressing α-synuclein (α-syn) or β-galactosidase (β-gal) were cultured in 15% normal human serum (NHS) or heat-inactivated human serum (HIS) for 10 days before viability was measured by an MTT assay. Viability was normalized to the control β-gal expression cells cultured in NHS. B Same as in A but cells were cultured in NHS without or in the presence of complement cascade inhibitors RaCI or Cp20. The bars represent the mean ± SD of three independent experiments. Statistical test: one-way ANOVA followed by Tukey’s multiple comparison test. n.s. not significant, *P < 0.05