Fig. 7From: TAK1 mediates neuronal pyroptosis in early brain injury after subarachnoid hemorrhageEffect of TAK1 knockdown on brain injury and inflammatory response after SAH. To knockdown of TAK1 in vivo, SAH mice were i.c.v injected with TAK1 siRNA. a Colocalization of Cy5-conjugated TAK1 siRNA with neurons (NeuN) at 24 h after SAH. b Western blotting and quantitative analysis for p-TAK1 and TAK1. c Modified Garcia score, d Beam balance test, and e brain water content in Sham, SAH+Vehicle, SAH+Scr siRNA, and SAH+TAK1 siRNA groups at 24 h after SAH. f–i Immunoblotting analysis and quantitation for NLRP3, ASC, cleaved caspase-1, IL-1β, GSDMD, nuclear p-NF-κB p65, and NF-κB p65 in treated mice. Data are expressed as mean ± SD, n = 5 in each group. ***P < 0.001 vs Sham group; ##P < 0.01, ###P < 0.001 vs SAH+Scr siRNA group. N.S., no significant difference. Scale bar: 50 μmBack to article page