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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: TAK1 mediates neuronal pyroptosis in early brain injury after subarachnoid hemorrhage

Fig. 9

OZ treatment inhibits lysosomal rupture in OxyHb-treated neurons. a Ctrl-, OxyHb-, and OxyHb+OZ-treated neurons were incubated with 100 nM Lyso-Tracker Red for 30 min to assess lysosomal stability. b Neurons of the three groups were incubated with 2 μg/ml Acridine Orange for 30 min to evaluate lysosomal dysfunction. c Quantitative analyses of the green fluorescence intensity of Acridine Orange with a microplate reader. d, e Western blot and quantitative analysis of lysosomal and total cathepsin B in the three groups. Data are expressed as mean ± SD, n = 5 per group. ***P < 0.001 vs Control group; ##P < 0.01, ###P < 0.001 vs OxyHb group. Scale bar: 50 μm

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