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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Cathepsin H deficiency decreases hypoxia-ischemia-induced hippocampal atrophy in neonatal mice through attenuated TLR3/IFN-β signaling

Fig. 2

Distribution and processing of CatH in microglia. A, B Immunofluorescent CLSM images of CatH (green) with Iba1, GFAP, and NeuN (red) in the ipsi hippocampus of WT mice 72 h after HI injury. Scale bar, 50 μm. Contra contralateral, ipsi ipsilateral. C Pulse-chase analysis of CatH in primary cultured microglia. The cells were labeled with [35S] methionine for 30 min and chased for the indicated time. At indicated time, cell lysate was prepared and used for immunoprecipitation of CatH. The immunoprecipitates were analyzed by SDS-PAGE and fluorography. D Immunofluorescent CLSM images of CatH (green) and Rab5 (red) in microglia. Scale bar, 10 μm

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