Fig. 4

CatH deficiency prevents the increased expression of p-STAT1 in microglia/macrophages. A, B CLSM images of Hoechst (blue), p-STAT1 (green, arrows), F4/80 (red), and their merged images in the cortex of WT (A) and CatH^−/− (B) mice 72 h after HI injury. Contra contralateral, ipsi ipsilateral. Scale bar, 50 μm. C The mean relative immunoreactivity for p-STAT1 in the pyramidal regions of the hippocampus of WT and CatH^−/− mice. The columns and bars represent the mean ± SD (n = 9). The asterisks indicate a statistically significant difference from contra (**p < 0.01, one-way ANOVA test). D The amount of released IFN-β from the MG6 microglia after OGD/R in the presence of cont or CatH siRNA. The columns and bars represent the mean ± SD (n = 3). The asterisks indicate a statistically significant difference from indicated group (**p < 0.01 and ***p < 0.001, one-way ANOVA test)