Fig. 5

CatH deficiency induces cell death of microglia/macrophages in ipsilateral hippocampus after HI injury. A, B Immunofluorescent CLSM images of Nissl (green) and Iba1 (red) in contra and ipsi cortex and hippocampus of WT (A) and CatH^−/− (B) mice 72 h after HI injury. Scale bar, 100 μm. Contra contralateral, ipsi ipsilateral. C The quantitative analyses of cell number of activated and non-activated microglial cells in WT and CatH^−/− mice 72 h after HI injury. The columns and bars represent the mean ± SD (n = 9). The asterisks indicate a statistically significant difference from WT ipsi group (***p <0.001, one-way ANOVA test). D CLSM images of Hoechst (blue) and Iba1 (red) in the hippocampus of WT and CatH^−/− mice 24 h after HI injury. Scale bar, 100 μm. E The mean relative immunoreactivity for Iba1 in the pyramidal regions of the hippocampus of WT and CatH^−/− mice. The columns and bars represent the mean ± SD (n = 9). The asterisks indicate a statistically significant difference from the WT. F The quantitative analyses of percentage of apoptotic MG6 cells after subjected to OGD/R. The columns and bars represent the mean ± SD (n = 3). The asterisks indicate a statistically significant difference from control group value (***p < 0.001, one-way ANOVA test). The daggers indicate a statistically significant difference from OGD/R value (^†††p < 0.001, one-way ANOVA test). G The quantitative analyses of percentage of apoptotic MG6 cells after subjected to OGD/R. The columns and bars represent the mean ± SD (n = 3). The asterisks indicate a statistically significant difference from control group value (***p < 0.01, one-way ANOVA test). The daggers indicate a statistically significant difference from OGD/R value (^††p < 0.01, one-way ANOVA test)