Fig. 1

Changes in gene expression in the rat DRG in 2 models of chronic pain. A Schematic of experimental setup. B PCA plot of RNA-seq samples. C Volcano plots of differential gene expression between CCI and naïve groups (left) and CFA and Naïve groups (right). Points highlighted in red indicated genes with differential expression as defined by an adjusted p value < 0.05 and a log₂fold change > |0.5|. D Dot plot comparing the significant biological processes identified from the lists of upregulated genes differential expressed in each pain group (i.e., CCI compared to naïve control, CFA compared to naïve control). The log q value for each term is indicated by color of each circle and the term enrichment (i.e., number of DEGs/total number of genes identified for that biological process) is indicated by size of the circle. E Heatmap indicating CCI- and CFA-specific genes with roles in pre-synaptic plasticity that were significantly upregulated in each pain model compared to naïve control. CCI chronic constriction injury, CFA complete Freund’s adjuvant, DEG differentially expressed gene