Fig. 4

Chromatin accessibility in the rat DRG after CCI and CFA. Volcano plots of DARs after CCI versus naïve groups (A) and after CFA versus Naïve (D). Points highlighted in red indicate genes with differential accessibility as defined by a p value < 0.001. Heatmap of read density for all DARs in CCI and naïve (B) and CFA and naïve (E) groups with increased accessibility (top panels) and decreased accessibility (bottom panels). Each row represents one accessible region and the regions are aligned at the center of each region. The color represents the intensity of chromatin accessibility. The average read density for each heatmap is shown to the right. C–F Gene ontology analysis of the molecular functions identified in DARs after CCI versus naïve (C) and CFA versus naïve (F). G Bar graph showing the proportion of DARs across genomic features after CCI or CFA. H Activity of candidate DRG enhancers by luciferase reporter gene assays. Individual reporter plasmids were prepared as described and contained one candidate enhancer regions (A–D; Supplemental Table 1). Luciferase activity was normalized to that of the Renilla reporter and expressed as mean fold relative activity of the empty reporter ± standard deviation. All constructs were tested in quadruplicate. CCI chronic constriction injury, CFA complete Freund’s adjuvant, DAR differentially accessible region