Fig. 2

Cell ferroptosis-conditioned medium induces proinflammatory cytokine production. a Flow cytometry analysis of C11-BODIPY oxidation in PC12 cells treated with RSL3 (1 μM) or RSL3 plus Fer (1 μM) for 10 h. b Cell viability or c LDH release was assessed and quantified for PC12 cells subjected to RSL3 (1 μM) treatment for 10 h. qRT-PCR analysis of TNF, IL-6, and IL-1b mRNA levels in primary microglia (d) or PMs (e) incubated with DMSO-treated cell culture supernatant or RSL3-treated cell culture supernatant for 8 h from PC12 cells. The data are means ± SD; for all panels, *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test. The data are combined from three independent experiments unless otherwise indicated