Fig. 4

RSL3 inhibits LPS-induced inflammation in a ferroptosis-independent manner. a The qRT-PCR analysis of IL-6 and IL-1b mRNA levels in LPS-stimulated microglia subjected to RSL3 (500 nM), RSL3 plus Fer-1 (1 μM) treatment for 4 h. b ELISA of IL-6 and IL-1β in supernatants of LPS (100 ng/ML) stimulated microglia subjected to RSL3, RSL3 plus Fer treatment for 8 h. c The qRT-PCR analysis of IL-6 and IL-1b mRNA levels in LPS-stimulated PMs subjected to RSL3, RSL3 plus Fer treatment for 4 h. d ELISA of IL-6 and IL-1β in supernatants of PMs subjected to RSL3, RSL3 plus Fer treatment for 8 h loading control. e Cell viability of primary microglia subjected to RSL3 (1 μM), RSL3 plus Fer treatment in the presence of LPS for 10 h. f Representative immunoblot analysis of GPX4 in BV2 microglia transfected with siNC or siGPX4 for 48 h. g The qRT-PCR analysis of TNF, IL-6, and IL-1b mRNA levels in LPS-stimulated BV2 microglia transfected with siNC or siGPX4 for 48 h and stimulated with LPS for 4 h. The data are means ± SD, for all panels: *P < 0.05, **P < 0.01, ***P < 0.001. n.s., no significant. a–e One-way-ANOVA analysis was performed. g Two-way-ANOVA analysis followed by Bonferroni post hoc test was performed. All data are representative of or combined from at least three independent experiments