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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Microglia and macrophage exhibit attenuated inflammatory response and ferroptosis resistance after RSL3 stimulation via increasing Nrf2 expression

Fig. 6

Knockdown of Nrf2 reverses ferroptosis resistance and inflammation suppression in response to RSL3 stimulation. Representative immunoblot analysis of Nrf2 in lysates of BV2 microglia (a) or PMs (b) subjected to RSL3 (500 nM) or DMSO treatment in the presence of LPS (100 ng/ML) for indicated times. Cell viability of BV2 microglia (c) or PMs (d) was assessed and quantified transfected with siNC or siNrf2 subjected to different doses of RSL3 treatment for 10 h. The qRT-PCR analysis of IL-6 and IL-1b in BV2 microglia (e) or PMs (f) transfected with siNC or siNrf2 in the presence or absence of RSL3. One-way-ANOVA analysis was used. The data are means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 by one-way-ANOVA analysis followed by Turkey test. n.s., no significant. All data are representative of or combined from at least three independent experiments

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