Fig. 4
From: Anti-AQP4 autoantibodies promote ATP release from astrocytes and induce mechanical pain in rats
Transcriptome profiling of the NMOSD pain model reveals IL-1β is a hub of molecular events. A PCA of transcriptome data obtained from spinal cords of rats receiving either control IgG or rAQP4 IgG. B Heat map representing expression levels of purinergic receptors. C Gene set enrichment analysis of genes upregulated in the spinal cord in the NMOSD pain model. The bar chart shows the top 10 enriched terms in WikiPathways 2016, along with their corresponding p-values. Colored bars correspond to terms with significant p-values. An asterisk next to a p-value indicates the term also has a significant adjusted p-value. D The protein–protein interaction network of the top 50 upregulated differentially expressed genes in spinal cords of rats receiving rAQP4 IgG, ranked by fold change. The graph was generated using the STRING database. The nodes represent differential genes and the number of edges reflects known protein–protein associations. Differently colored edges indicate different types of evidence used in predicting interactions. Red line indicates the presence of fusion evidence. Green line indicates neighborhood evidence. Blue line indicates cooccurrence evidence. Purple line indicates experimental evidence. Yellow line indicates textmining evidence. Light blue line indicates database evidence. Black line indicates coexpression evidence. E Gene expression of IL1B in rat spinal cord of either rAQP4 IgG or control IgG–treated group. Data are expressed as means ± SEM, and were analyzed by Student’s t-test and *P < 0.05