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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Repeated neonatal sevoflurane induced neurocognitive impairment through NF-κB-mediated pyroptosis

Fig. 4

BAY 11-7082 attenuates sevoflurane-induced pyroptosis in primary hippocampal neurons. A Representative western blotting and quantitative analysis of the protein levels of GSDMD and GSDMD-N from primary hippocampal neuron homogenates obtained on DIV 11. B Representative immunofluorescence staining images of MAP2, GSDMD, and DAPI in primary hippocampal neurons. Scale bars = 50 μm. C Quantitative analysis of the intensity of GSDMD-immunofluorescence in primary hippocampal neurons. Values are presented as mean ± SEM (n = 6 cultures/group). *p < 0.05 versus the Con group; #p < 0.05 versus the Sev group

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