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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Attenuating vascular stenosis-induced astrogliosis preserves white matter integrity and cognitive function

Fig. 8

Bulk RNA-seq analysis of transcriptome changes in brain astrocytes. a Schematics of brain astrocyte isolation for bulk RNA-seq analysis from sham, BCAS+Veh (i.p.), and BCAS+HOE642 (i.p.) mice at 30 days post-surgery. n = 4/group. b Venn diagram depicting differential gene expression in brain astrocytes (p value ≤ 0.05 and FC≥1.5). c Volcano plots illustrate the gene expression pattern detected with p value ≤ 0.05 and FC≥1.5. Red dot: significant differentially expressed gene; Blue dot: non-significant differentially expressed gene. d Comparison analysis of significantly altered canonical pathways by ingenuity pathway analysis (IPA) between BCAS+Veh vs. sham group and BCAS+HOE vs. BCAS+Veh group. p value < 0.05 and Z-score ≥ 3. e ROS production genes expression. Data are RPKM values shown as mean ± SD, n = 3-4. *p ≤ 0.05 and FC >1.5. f Inflammatory gene expression. Data are RPKM values shown as mean ± SD, n = 3–4. *p ≤ 0.05 and FC > 1.5

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