Fig. 1
From: Sex differences and similarities in the neuroimmune response to central administration of poly I:C
Housekeeping gene stability analysis. A Distribution of the quantification cycles (Cq) for housekeeping genes 18s, gapdh, hprt1, and rplp0, with B associated standard deviations. C Distribution of the difference of Cq values (ΔCq) between pairs of housekeeping genes, and D the associated standard deviations. E Stability values calculated using gene ratio method by Vandesompele et al. 2002, which uses stepwise elimination of lowest stability (highest M value) to rank gene stability. F Stability values calculated using a model-based approach by Andersen et al. 2004 which measures expression variation such that highest stability results in the lowest Rho value. G Summary of results from each of the four methods of housekeeping gene stability are shown