Table 1 Primer sequences used for real-time PCR
From: Sex differences and similarities in the neuroimmune response to central administration of poly I:C
Gene target | Forward primer sequence (5′ to 3′) | Reverse primer sequence (5′ to 3′) | NCBI reference sequence |
|---|---|---|---|
ccl2 | CCACAACCACCTCAAGCACT | AAGGCATCACAGTCCGAGT | NM_011333.3 |
cd11b | CGTGAATGGGGACAAACTGAC | GCACTGAGGCTGGCTATTGA | NM_008401.2 |
cxcl10 | TCCATCACTCCCCTTTACCCA | TGGCTTGACCATCATCCTGC | NM_021274.2 |
gfap | AAACCGCATCACCATTCCTG | CCCGCATCTCCACAGTCTTTA | NM_010277.3 |
ifnα | AGAGAAGAAACACAGCCCCT | AGCACATTGGCAGAGGAAGA | NM_010502.2 |
ifnβ | GCTCCAAGAAAGGACGAACAT | GGATGGCAAAGGCAGTGTAA | NM_010510.1 |
ifnγ | GTCAACAACCCACAGGTCCA | CGACTCCTTTTCCGCTTCCT | NM_008337.4 |
il-1β | TGCCACCTTTTGACAGTGATG | GCTCTTGTTGATGTGCTGCT | NM_008361.4 |
il-6 | GAGACTTCCATCCAGTTGCCT | TCATTTCCACGATTTCCCAGAG | NM_001314054.1 |
il-10 | CTGGACAACATACTGCTAACCG | AATGCTCCTTGATTTCTGGGC | NM_010548.2 |
tnfα | ACCCCTTTACTCTGACCCCTT | ACTGTCCCAGCATCTTGTGT | NM_001278601.1 |