Fig. 10

Inhibition of TNFR1 reduces RGC apoptosis in TNF-α injected and COH retinas. a Representative images of TUNEL staining detection of apoptotic RGCs in Ctr (a1), TNF-α3D (a4), R7050+TNF-α3D (a7), TNF-α7D (a10), R7050+TNF-α7D (a13), COH2W (a16), and R7050+COH2W (a19) groups. R7050 (10 μM, 2 μl) was intravitreally injected 1 day before TNF-α injection or COH operation. All images were taken from the whole-flat mounted retinas in the regions at angle 0°. a2, a5, a8, a11, a14, a17, and a20 were DAPI images. Merged images of TUNEL and DAPI were showed in a3, a6, a9, a12, a15, a18, and a21. Scale bar = 50 μm for all images. b Bar charts summarize the average number of TUNEL-positive signals in whole flat-mounted retinas in different groups. n = 5–7. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. Ctr; ^###P < 0.001