Fig. 6

The inhibition of P2X4R alleviates white matter injury after ICH. A Representative images of double immunofluorescence staining for NF200 and MBP in the peri-hematoma region on the third day after ICH. B Mean fluorescence densities of MBP after 3 days of ICH (n = 5 per group). C–E Representative western blot images show the protein expression of MBP and APP in the hematoma region in the 5-BDBD treatment groups compared with the vehicle group 3 days after ICH (n = 5 per group). F Double immunofluorescence staining for MBP and NF200 in the peri-hematoma region 7 days after ICH. G Mean fluorescence densities of MBP 7 days after ICH (n = 5 per group). H–J Representative western blot images show the protein expression of MBP and APP in the hematoma region in the 5-BDBD treatment groups compared with the vehicle group 7 days after ICH (n = 5 per group). Data are expressed as the means ± SEM. *P < 0.05. **P < 0.01. ***P < 0.001 vs. ICH + vehicle group. ^#P < 0.05. ^##P < 0.01. ^###P < 0.001 vs. ICH + 5-BDBD group. Scale bar = 100 μm