Fig. 1

Comparison of immune effector secretion by postnatal and adult microglia when exposed to TLR agonists ex vivo. Equal numbers of microglia isolated from postnatal (P3) and adult (A) mice were exposed to LPS, IMQ, CpG-ODN 1585 (CpG), or medium alone (Med) for 10 h before harvesting. Secreted immune effectors were measured in the harvested medium by Bio-Plex suspension array system. Each dot indicates an individual well of microglia that was isolated from either 6 (adults) or 4 (P3) mice and cultured separately. Statistical analysis was performed using a one-way ANOVA with an uncorrected Fisher’s least significant difference test to compare P3 to adult microglia exposed to TLR agonists. ****P ≤ 0.0001; ***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.05. ns not significant