Fig. 4
Determination of microglia in the adult mouse brain with and without PLX5622 treatment after acute TLR agonist or PBS exposure. Mice were either treated with PLX5622 (+) or untreated (−) for 28 days prior to a 6-h exposure to LPS, IMQ, CpG-ODN 1585 (CpG), or PBS as a control. Similar paraffin-embedded sections of cortex and thalamus were fixed and stained with antibody to Iba1. Panels A–D are representative images of a brain from a mouse either treated with PLX5622 (A and C) or untreated (B and D). IBA1-positive (IBA+) cells were enumerated by microscopic examination of the cortex (E) and thalamus (F) and reported as the number of positive cells per square millimeter. The white columns represent the mean number of IBA+ cells in untreated mice, and the gray columns represent the mean numbers of IBA+ cells in mice treated with PLX5622. Each dot represents an individual mouse. Error bars represent 1 standard deviation. Statistical analysis was performed using a Student’s t test on the mean IBA+ cells per mm2 to compare PLX5622-treated (reduced microglia) to untreated mice. ****P ≤ 0.0001