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Table 5 TLR signaling pathway associated genes significantly altered in the brain of untreated mice after 6 h of exposure to TLR agonists relative to PBS-treated control mice (n = 3)

From: Innate immune responses after stimulation with Toll-like receptor agonists in ex vivo microglial cultures and an in vivo model using mice with reduced microglia

LPS (n = 4) IMQ (n = 4) CpG-ODN 1585 (n = 3)
Gene FC P value Gene FC P value Gene FC P value
Cxcl10 500.5 < 1.0E-10 Cxcl10 182.7 < 1.0E-10 Cxcl10 873.3 < 1.0E-10
Ccl2 110.3 < 1.0E-10 Ccl2 28.6 < 1.0E-10 Ccl2 182.9 < 1.0E-10
Il6 65.8 < 1.0E-10 Tnf 14.0 < 1.0E-10 Il6 108.0 < 1.0E-10
Csf3 51.2 < 1.0E-10 Il6 10.8 < 1.0E-10 Tnf 72.9 < 1.0E-10
Il1b 49.5 < 1.0E-10 Tlr2 4.2 2.0E-07 Il1b 69.7 < 1.0E-10
Tnf 42.0 < 1.0E-10 Il1b 3.8 1.1E-06 Clec4e 68.2 < 1.0E-10
Clec4e 29.0 < 1.0E-10 Eif2ak2 3.3 1.4E-05 Csf3 32.5 < 1.0E-10
Il1a 17.1 < 1.0E-10 Irf1 2.6 4.0E-04 Tlr2 21.0 < 1.0E-10
Tlr2 16.4 < 1.0E-10 Csf3 2.6 4.8E-04 Cd14 12.6 < 1.0E-10
Cd14 11.9 < 1.0E-10 Tlr1 2.5 6.4E-04 Il1a 10.6 < 1.0E-10
Tlr1 7.1 < 1.0E-10 Il1a 2.2 3.3E-03 Il10 7.6 2.1E-09
Il10 6.8 < 1.0E-10 Myd88 2.1 5.8E-03 Tlr1 6.9 1.1E-08
Nfkbia 5.5 1.5E-09 Cd14 2.1 5.8E-03 Eif2ak2 6.0 9.1E-08
Eif2ak2 4.3 2.1E-07 Ifnb1 − 4.4 7.9E-08 Irf1 5.8 1.7E-07
Myd88 4.1 4.6E-07 Csf2 − 82.5 < 1.0E-10 Ifnb1 5.5 3.7E-07
Tnfaip3 4.0 8.6E-07     Myd88 4.9 1.9E-06
Irf1 3.8 1.4E-06     Nfkbia 4.4 8.0E-06
Cd86 3.7 2.3E-06     Tnfaip3 3.7 8.2E-05
Cd80 3.1 3.8E-05     Tlr3 3.0 8.9E-04
Nfkb2 2.7 3.1E-04     Tlr8 3.0 9.6E-04
Ptgs2 2.7 3.1E-04     Cd80 2.8 2.0E-03
Ifng 2.6 7.2E-04     Nfkb2 2.8 2.1E-03
Tnfrsf1a 2.4 1.5E-03     Cd86 2.6 4.7E-03
Ripk2 2.3 2.0E-03     Ripk2 2.5 5.7E-03
Cebpb 2.3 2.8E-03     Casp8 2.5 5.7E-03
Il1r1 2.1 7.2E-03     Tnfrsf1a 2.5 6.4E-03
Tlr3 2.1 6.7E-03     Ptgs2 2.4 7.3E-03
Pglyrp1 2.0 9.7E-03     Tlr6 2.3 1.0E-02
Fos − 2.0 9.7E-03     Muc13 2.0 3.1E-02
Ifnb1 − 3.1 4.7E-05     Nfkbil1 − 2.1 2.5E-02
Csf2 − 49.4 < 1.0E-10     Csf2 − 43.7 < 1.0E-10
  1. n is the number of independent mouse RNA samples tested. Fold change (FC) relative to PBS treated mice. P values are calculated using the student t-test on the Delta CT. All changes reached our criteria of statistical significance where the FC is greater or less than 2-fold with a P value ≤ 5.0E-02