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Table 6 TLR signaling pathway associated genes altered in the brain of PLX5622-treated relative to Untreated mice after 6 hours of exposure to TLR agonists or PBS

From: Innate immune responses after stimulation with Toll-like receptor agonists in ex vivo microglial cultures and an in vivo model using mice with reduced microglia

  PLX5622-treated (n = 3) relative to Untreated exposed to PBS (n = 3) PLX5622-treated (n = 4) relative to Untreated exposed to LPS (n = 4) PLX5622-treated (n = 4) relative to Untreated exposed to IMQ (n = 4) PLX5622-treated (n = 3) relative to Untreated exposed to CpG-ODN 1585 (n = 3)
Gene FC P value FC P value FC P value FC P value
Btk − 2.2 8.5E-03 − 4.2 5.2E-07 − 3.0 2.8E-04 − 2.3 1.1E-02
Casp8 − 1.3 3.5E-01 − 1.8 4.6E-02 − 2.0 2.0E-02 − 2.9 1.0E-03
Ccl2 − 1.4 2.6E-01 − 1.0 9.8E-01 − 8.0 < 1.1E-10 − 1.6 1.6E-01
Cd14 − 2.6 1.4E-03 − 3.0 1.2E-04 − 3.9 8.9E-06 − 4.1 1.6E-05
Cd80 − 1.5 2.1E-01 − 1.4 2.4E-01 − 1.4 2.6E-01 − 2.2 1.9E-02
Cd86 − 6.1 4.9E-09 − 13.6 < 1.1E-10 − 5.1 9.7E-08 − 11.0 < 1.1E-10
Clec4e 1.3 4.0E-01 − 20.5 < 1.1E-10 − 1.3 4.2E-01 − 3.4 1.6E-04
Csf2 − 1.7 6.6E-02 2.3 4.4E-03 2.2 1.0E-02 − 1.5 2.5E-01
Csf3 1.2 5.3E-01 1.6 8.3E-02 − 2.4 3.9E-03 − 3.9 3.4E-05
Cxcl10 1.8 6.0E-02 − 1.4 2.8E-01 − 13.2 < 1.1E-10 − 2.6 3.4E-03
Ifnb1 1.2 5.0E-01 1.2 5.3E-01 1.3 3.6E-01 − 2.2 1.4E-02
Ifng 2.5 3.0E-03 1.2 5.6E-01 1.0 9.8E-01 4.4 6.8E-06
Il10 1.2 4.8E-01 − 4.0 1.5E-06 1.1 8.7E-01 − 3.2 4.3E-04
Il1a − 1.5 2.1E-01 − 5.8 1.1E-09 − 2.9 5.1E-04 − 7.0 4.3E-09
Il1b − 1.1 6.6E-01 − 4.1 7.5E-07 − 2.9 5.1E-04 − 2.1 2.0E-02
Il6 − 1.6 1.2E-01 − 1.6 8.8E-02 − 5.2 7.6E-08 − 2.0 3.2E-02
Irf1 1.3 4.2E-01 1.2 4.9E-01 − 2.0 2.4E-02 − 1.6 1.3E-01
Ly86 − 3.5 4.8E-05 − 10.7 < 1.1E-10 − 3.2 1.5E-04 − 4.3 1.0E-05
Nfkbib 1.2 6.5E-01 − 2.0 1.6E-02 − 1.1 8.2E-01 1.5 1.9E-01
Ticam2 − 3.8 1.2E-05 − 8.7 < 1.1E-10 − 5.5 2.5E-08 − 2.7 2.7E-03
Tlr1 − 7.4 1.0E-10 − 28.1 < 1.1E-10 − 7.3 < 1.1E-10 − 12.0 < 1.1E-10
Tlr2 1.2 6.3E-01 − 2.1 8.6E-03 − 3.4 5.3E-05 − 3.2 4.0E-04
Tlr5 − 1.3 4.5E-01 − 1.3 4.2E-01 − 2.9 4.3E-04 − 1.8 6.7E-02
Tlr6 − 3.4 5.8E-05 − 5.2 1.1E-08 − 1.7 9.8E-02 − 5.4 3.5E-07
Tlr7 − 4.3 2.1E-06 − 17.1 < 1.1E-10 − 5.6 1.7E-08 − 6.8 6.2E-09
Tlr8 − 4.6 7.6E-07 − 13.5 < 1.1E-10 − 12.6 < 1.1E-10 − 1.7 1.2E-01
Tlr9 − 1.8 6.3E-02 − 1.2 4.8E-01 − 2.6 1.7E-03 − 1.4 2.6E-01
Tnf 1.6 1.1E-01 − 10.1 < 1.1E-10 − 11.4 < 1.1E-10 − 4.5 4.6E-06
  1. n is the number of independent mouse RNA samples tested. FC is fold change in gene expression as indicated. P values are calculated using the student t-test on the Delta CT. All changes in bold reached our criteria of statistical significance where FC is greater or less than 2.0-fold with a P value ≤ 5.0E-02