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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Asthmatic allergen inhalation sensitises carotid bodies to lysophosphatidic acid

Fig. 3

Lysophosphatidic acid receptors are upregulated in asthmatic rats. qPCR reveals that lysophosphatidic acid receptor (LPAr) 1, 2, 4, 5, 6 mRNA was upregulated in the carotid bodies (a), and petrosal ganglia (b) of ovalbumin sensitised and challenged (OVA) rats compared to naïve Brown Norway (BN) rats. TRPV1 was not detected in the carotid body (a) as demonstrated previously [15] and was not increased in OVA compared to naïve BN rats in the petrosal ganglion (b). PKCε was detected in all tissues analysed but was not increased in OVA compared to naïve BN rats (a-b). c LPAr 1, 2, 4, 5, 6 were only detected after 45 cycles in OVA but not naïve BN rats in the superior cervical ganglion. d No discernible increases in LPAr expression were revealed in the nodose ganglion. TRPV1 was detected in the superior cervical ganglion (c) and nodose ganglion (d) but was not increased in OVA compared to naïve BN rats. In OVA rats, the carotid body (e) and petrosal ganglion (f) demonstrated increased LPAr and TRPV1 mRNA expression in comparison to the nodose ganglion. Differences in expression were analysed by the 2∆ctt method [17] in reference to hypoxyribosyltransferase (HPRT) housekeeping gene. The 2∆ctt number indicating an increase in of red verses black bars are expressed above bars for specific receptor

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