Fig. 3

Hypothalamic transcript expression of IL6. In order to identify obese-prone (H) and obese-resistant (L) mice, we performed the procedure as depicted in A. The expression of hypothalamic IL6 was determined using real-time PCR (B). Palmitate was injected in a single intracerebroventricular (ICV) dose and hypothalamus was collected (C) for determinations of IL6 (D), TNFα (E), and IL1β (F) transcripts. B, n = 7–8 mice/group; D–F, n = 5 mice/group; *p < 0.05, **p < 0.01, ***p < 0.001. In bar graphs, results are presented as mean ± standard deviation