Fig. 4

IL6 effect on hypothalamic cell proliferation and survival. Mice were treated with IL6 and BrdU according to the protocols as depicted in A and D. The mediobasal hypothalamus was prepared for analysis using immunofluorescence and confocal microscopy; cell counting was performed using 20× and 40× magnifications. Representative images are presented in B (proliferation assay) and E (survival assay). Total BrdU countings are presented in C (proliferation assay) and F (survival assay). In both assays, n = 6 mice/group. In bar graphs, results are presented as mean ± standard deviation