Fig. 7

IL6 induce neurogenic factors expression in hypothalamic neuroprogenitor cells (NPCs). Transcripts were determined using real-time PCR in the NPCs of WT and IL6 KO mice after a 5-day differentiation period: first, WT and IL6 KO hypothalamic cells were analyzed in basal conditions (A), and then, IL6 KO NPCs were treated with exogenous IL6 in doses depicted (B). Representative immunocytochemistry images of 7-day differentiated cells (green, DCX; red, GFAP; blue, DAPI) (C) and DCX-positive cell counting (D), and representative immunocytochemistry images of 14-day differentiated cells (green, MAP2; red, GFAP; blue, DAPI) (E) and MAP2-positive cell counting (F). After 10- (G, I, K–M) or 14-day (H and J) differentiation period, transcripts were determined using real-time PCR in the NPCs of WT and IL6 KO mice treated with exogenous IL6 in doses depicted in the panels (G-M). A, B n = 12 (3–4 well/group, 2 independent experiments); C–F n = 24 (4 well/group, 2 independent experiments); G–M n = 48 (6–9 well/group, 3 independent experiments). *p < 0.05, **p < 0.01. In bar graphs, results are presented as mean ± standard deviation