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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Galantamine prevents and reverses neuroimmune induction and loss of adult hippocampal neurogenesis following adolescent alcohol exposure

Fig. 1

Experimental timeline. Two separate experiments were conducted to test whether galantamine can (1) prevent or (2) reverse AIE-induced deficits in hippocampal pathology. These studies were conducted in male Wistar rats. Between P25 and 54 in both studies, rats underwent a 2-day on/2-day off schedule of intragastric gavage with either 5 g/kg ethanol (AIE) or water (CON). In the prevention study (study 1, upper timeline), 4 mg/kg galantamine (Gal) (indicated by upper clue dots) or saline (Veh) was administered intraperitoneally as a pretreatment 30 min prior to ethanol/water gavage (indicated by upper red dots), i.e., 16 doses across 30 days. Treatment ended on P54 and rats were allowed to rest in their home cage until P70, after which they were euthanized and their brains harvested for IHC. In the restoration study (study 2, bottom timeline), rats completed the AIE paradigm (indicated by lower red dots) and then starting on P57 were administered intraperitoneal injections of either Gal or Veh (indicated by lower blue dots). Rats underwent 14 daily injections and then were euthanized on P73 when their brains were harvested for either IHC or western blotting (WB), respectively. In study 1, n = 42. In study 2, n = 39 for IHC, n = 29 for WB

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