Fig. 8

Galantamine increases Ki67 but not PCNA in the subgranular zone of the dentate. (A) Ki67 is a fairly specific marker of cell proliferation which is upregulated in the active phase of the cell cycle. Similar to the prevention study, we found that 2 weeks of galantamine treatment increased Ki67+IR in the subgranular zone, independent from adolescent exposure to ethanol or water. (B) Representative photomicrographs were taken of dentate Ki67+IR from each group (20× magnification), demonstrating that galantamine treatment increases Ki67+IR, regardless of adolescent exposure condition. (C) While PCNA is expressed during cell proliferation, it is also expressed when DNA is undergoing cell repair. Here, we found that AIE decreased PCNA+IR relative to water-treated controls in vehicle-treated cohorts. The divergence of these findings from Ki67 could suggest that AIE is decreasing the induction of appropriate cell repair machinery in response to DNA damage. (D) Photomicrographs of PCNA+IR in the dentate gyrus. A representative photomicrograph of the hippocampal dentate gyrus was taken at 20× magnification for each group. Results are expressed as mean ± SEM. Scale bars indicate 100 μM. For each identified comparison, *p < 0.05