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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: MD2 contributes to the pathogenesis of perioperative neurocognitive disorder via the regulation of α5GABAA receptors in aged mice

Fig. 3

MD2 knockdown by AAV-shMD2 ameliorates anesthesia and surgery-induced cognitive decline. A Schematic timeline of virus injection and behavioral test. B Schematic diagram of bilateral virus injections. C The expression of MD2 in the hippocampus determined by immunofluorescence assay 3 weeks after the virus injection. D Statistical analysis of mean immunofluorescence intensity (p = 0.0117; n = 4 each). Scale bar = 100 μm. E Hippocampal MD2 expression determined by western blot analysis (p = 0.037; n = 3 each). Tubulin was used as a loading control. F Representative tracks and heat maps of the mice in the Barnes maze. G Mean latency in the training phase (F(1,15) = 8.034, p = 0.012. post hoc: day 5 p = 0.430, day 6 p = 0.048, day 7 p = 0.083, day 8 p = 0.661). H Time spent in the target zone in the probe phase (p = 0.0093). I Average speed in the probe phase (p = 0.3296). J Total distance in the probe phase (p = 0.8839). The data are presented as the mean ± SD; * p < 0.05, compared with the shCon group or Surgery + shCon group, ** p < 0.01, compared with the Surgery + shCon group, n = 8 Surgery + shCon, n = 9 Surgery + shMD2

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