Fig. 5
Effect of MD2 on the expressions of total α5GABAARs and membrane α5GABAARs and the tonic currents in CA1 pyramidal neurons in the hippocampus. A The effect of TCM treatment on the expression of total α5GABAARs in the cultured primary neurons (p = 0.0081; n = 3 each). B The effect of TCM treatment on the expression of membrane α5GABAARs in the cultured primary neurons (p = 0.0056; n = 3 each). C The expression of total MD2 in the hippocampus of mice (F(2,6) = 17.98, p = 0.0029. * p = 0.0342, Sham vs. Postsurgery; ## p = 0.0024, Postsurgery vs. Postsurgery + TCM; n = 3 each). D The expression of membrane α5GABAARs in the hippocampus of mice in the Sham, Postsurgery, and Postsurgery + TCM groups (F(2,15) = 6.946, p = 0.0073. * p = 0.0462, Sham vs. Postsurgery; ## p = 0.007, Postsurgery vs. Postsurgery + TCM; n = 6 each). Sodium-potassium ATPase (NKA) was used as a loading control. E The effect of LPS on the expression of total α5GABAARs in the hippocampus (p = 0.8197; n = 4 each). F The effect of LPS on membrane α5GABAARs expression in the hippocampus (p = 0.0435; n = 8 each). G, H TCM decreased the tonic currents generated by α5GABAARs in slices of the hippocampal CA1 subfield (p = 0.0158; n = 8 Saline, n = 6 TCM). I TCM had no effect on the sIPSC in slices of the hippocampal CA1 subfield. J Frequency of sIPSC (p = 0.8165; n = 8 Saline, n = 6 TCM). K Amplitude of sIPSC (p = 0.7839; n = 8 Saline, n = 6 TCM). The data are presented as the mean ± SD; * p < 0.05, compared with the Sham group, ACSF group, or Saline group; ** p < 0.01, compared with the Saline group; ## p < 0.01, compared with the Postsurgery group