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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Rapid morphologic changes to microglial cells and upregulation of mixed microglial activation state markers induced by P2X7 receptor stimulation and increased intraocular pressure

Fig. 3

Isolated microglial cells express P2X7 receptors on protein and functional levels. a Immunostaining of the P2X7 receptor (green) in primary retinal microglial cells stained positive for Iba1 (red). Bar = 10 μm. b Representative trace from a retinal microglia cell loaded with Fura-2 showing elevation in cytoplasmic Ca2+ in response to BzATP (100 μM, Mg2+ free solution). Data displayed as the ratio of light excited at 340/380 nm, emitted > 520 nm (referred to as “F(340/380)”). The response to BzATP was transiently reduced by 1 μM P2X7 antagonist A839977 (A83). c Quantification of the response in multiple cells (RM 1-way ANOVA with Sidak’s MC test; n = 12 cells from 3 culture preparations)

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Journal of Neuroinflammation

ISSN: 1742-2094