Fig. 3From: Rapid morphologic changes to microglial cells and upregulation of mixed microglial activation state markers induced by P2X7 receptor stimulation and increased intraocular pressureIsolated microglial cells express P2X7 receptors on protein and functional levels. a Immunostaining of the P2X7 receptor (green) in primary retinal microglial cells stained positive for Iba1 (red). Bar = 10 μm. b Representative trace from a retinal microglia cell loaded with Fura-2 showing elevation in cytoplasmic Ca2+ in response to BzATP (100 μM, Mg2+ free solution). Data displayed as the ratio of light excited at 340/380 nm, emitted > 520 nm (referred to as “F(340/380)”). The response to BzATP was transiently reduced by 1 μM P2X7 antagonist A839977 (A83). c Quantification of the response in multiple cells (RM 1-way ANOVA with Sidak’s MC test; n = 12 cells from 3 culture preparations)Back to article page