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Table 1 List of antibodies used for immunofluorescent staining

From: Rapid morphologic changes to microglial cells and upregulation of mixed microglial activation state markers induced by P2X7 receptor stimulation and increased intraocular pressure

Antibody

Source

Identifier

Dilution

Use

Primary antibodies

 Anti-Brn-3a

Santa Cruz

Cat# sc-31984

1:250

Whole mounts

 Anti-GFAP

Chemicon

Cat# MAB360

1:500

Immunocytochemistry

 Anti-Iba1

Wako

Cat# 019-19741

1:500

Whole mounts, cryosections, immunocytochemistry

 Anti-Iba1

Abcam

Cat# AB48004

1:200

Immunocytochemistry

 Anti-P2X7 Receptor

Alomone

Cat# APR-008

1:200

Immunocytochemistry

 Anti-Synaptophysin

Thermo Fisher

Cat# MA5-14532

1:250

Immunocytochemistry

Secondary antibodies

 Anti-Mouse Alexa Fluor 488

Thermo Fisher

Cat# A21202

1:500

Whole mounts

 Anti-Rabbit Alexa Fluor 488

Thermo Fisher

Cat# A21206

1:500

Immunocytochemistry

 Anti-Mouse Alexa Fluor 488

Thermo Fisher

Cat# A11001

1:500

Immunocytochemistry

 Anti-Goat Alexa Fluor 488

Thermo Fisher

Cat# A11055

1:500

Immunocytochemistry

 Anti-Rabbit Alexa Fluor 546

Thermo Fisher

Cat# A11035

1:500

Immunocytochemistry

 Anti-Goat Alexa Fluor 555

Thermo Fisher

Cat# A21432

1:500

Immunocytochemistry

 Anti-Rabbit Alexa Fluor 555

Thermo Fisher

Cat# A31572

1:500

Whole mounts, immunocytochemistry

 Anti-Goat Alexa Fluor 555

Thermo Fisher

Cat# A10042

1:500

Cryosections