Western blots confirm IL-10, IL-6, and IL-1β expression increase with age in mouse ventral hippocampus. A An IL-10 antibody detected a doublet migrating at ~25 kDa, possibly due to detection of differentially glycosylated forms. Across sexes, old and middle-aged mice showed higher expression of the bottom band relative to young adult mice. The fact that the effect of age occurs across sexes is indicated by the significance marker (i.e., * or #) being placed above a bar that extends across the male and female histograms (see Table 1). B IL-6 (migrating as a single band at ~21 kDa) was also elevated in old mice relative to young adult and middle-aged mice. This age-related increase was observed across males and females, but was smaller in males. C Across sexes, IL-1β protein (migrating as a doublet at ~25 kDa possibly due to detection of differentially glycosylated forms) was also elevated in old versus young adult mice. Data expressed as mean ±SEM. Brightness and contrast of blots and Ponceau stain (PS) images adjusted for graphical clarity. Effect of age across sexes: *vs. young, P=0.049 to <0.00001; #vs. young and middle-aged, P<0.001. Rank Sum test of sex within old: @vs. female, P=0.013. LB—lower band, UB—upper band, Y—young, M—middle aged, O—old.