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Table 1 Statistics and n’s for experiments shown in Figs. 1, 2, 3, 4, and 5

From: Aging triggers an upregulation of a multitude of cytokines in the male and especially the female rodent hippocampus but more discrete changes in other brain regions

  YM YF MM MF OM OF     
Figure    n=    Normality EVa effect of age Effect of sexb
1A 4 3    4 3 Pass Fail Rank sum: T(7,7)=35.00, FDR-P=0.036  
1B 4 3    4 3 Pass Fail Rank sum: T(7,7)=30.50, FDR-P=0.013  
1C 4 3    4 3 Pass Pass Student’s t: t(12)=-3.472, FDR-P=0.019  
1D 4 3    4 3 Pass Pass Student’s t: t(12)=-2.375, FDR-P=0.044  
1E 4 3    4 3 Pass Pass Student’s t: t(12)=-3.128, FDR-P=0.023  
1F 2 1    2 1 Pass Pass Student’s t: t(4)=-1.53, FDR-P=0.178  
1G 4 3    4 3 Pass Fail Rank sum: T(7,7)=31.00, FDR-P=0.019  
1H 4 3    4 3 Pass Pass Student’s t: t(12)=-2.340, FDR-P=0.044  
1I 4 3    4 3 Fail   Rank sum: T(7,7)=32.00, FDR-P= 0.023  
1J 4 3    4 3 Fail   Rank sum: T(7,7)=32.00, FDR-P=0.023  
1K 4 3    4 3 Pass Pass Student’s t: t(12)=-5.641, FDR-P=0.002  
1L 4 3    4 3 Fail   Rank sum: T(7,7)=28.50, FDR-P=0.007  
1M 4 3    4 3 Pass Fail Rank sum: T(7,7)=34.00, FDR-P=0.029  
1N 4 3    4 3 Fail   Rank sum: T(7,7)=28.00, FDR-P=0.007  
1O 4 3    4 3 Fail   Rank sum: T(7,7)=33.50, FDR-P=0.025  
1P 4 3    4 3 Pass Pass Student’s t: t(12)=−1.052, FDR-P=0.314  
1Q 4 3    4 3 Pass Pass Student’s-t: t(12)=-2.386, FDR-P=0.044  
1R 4 3    4 3 Pass Pass Student’s t: t(12)=-2.996, FDR-P=0.025  
1S 4 3    4 3 Fail   Rank sum: T(7,7)=41.00, FDR-P=0.179  
1T 4 3    4 3 Pass Pass Student’s t: t(12)=-2.554, FDR-P=0.036  
1U 4 3    4 3 Fail   Rank sum: T(7,7)=33.00, FDR-P=0.025  
1V 4 3    4 3 Pass Pass Student’s t: t(12)=-2.836, FDR-P=0.027  
1W 4 3    4 3 Pass Fail Rank sum: T(7,7)=34.50, FDR-P=0.026  
2A-left 7 7 4 4 8 6 Fail   ANOVA on ranks: H(2)=1.51, P=0.471 Rank sum: T(6,8)=47.0, P=0.852
2A-right 7 7 4 4 8 6 Pass Fail ANOVA on ranks: H(2)=8.505, P=0.014c Rank sum: T(5,8)=41.0, P=0.435
2B 13 13 17 16 16 13 Fail   ANOVA on ranks: H(2)=44.227, P<0.001c Rank sum: T(13,16)=252.00, P=0.013
2C-left 9 5    9 4 Pass Fail Rank sum: T(13,14)=226.00, P=0.035 Rank sum: T(4,9)=31.0, P=0.70
2C-right 9 5    9 4 Pass Fail Rank sum: T(13,14)=247.00, P=0.002 Rank sum: T(4,9)=30.0, P=0.817
3A 9 8 12 11 17 14 Fail   ANOVA on Ranks: H(2) = 50.059, P<0.001c Rank sum: T(14,17)=230.0, P=0.827
3B-left 6 5    5 5 Pass Pass 2-Way ANOVA: F(1,17)=11.207, P=0.004 2-Way ANOVA: F(1,17)=0.01, P=0.928
3B-right 6 5    5 5 Pass Pass 2-Way ANOVA: F(1,17)=11.443, P=0.004 2-Way ANOVA: F(1,17)=0.003, P=0.955
3C 15 12    9 14 Fail   Rank sum: T(23,27)=289.0, P<0.001 Rank sum: T(4,8)=23.0, P=0.683
3D-left 2 3    2 3 Pass Pass Student’s t: t(8)=0.43, P=0.677  
3D-right 2 3    2 3 Pass Pass Student’s t: t(8)=0.18, P=0.862  
3E 16 13    10 10 Pass Pass 2-Way ANOVA: F(1,45)=74.93, P<0.001 2-Way ANOVA: F(1,16)=0.22, P=0.649
3F-left 11 6   11 7 Fail    Rank sum: T(17,18)=270.5, P=0.248 Rank sum: T(7,11)=50.0, P=0.147
3F-right 11 7   11 7 Pass Fail   Rank sum: T(18,18)=328.0, P=0.887 Rank sum: T(7,11)=46.0, P=0.07
3G 14 13 16 17 16 16 Fail   ANOVA on ranks: H(2)=53.334, P<0.001c Rank sum: T(15,16)=207.0, P=0.199
3H 9 9 4 4 9 9 Pass Pass 2-Way ANOVA: F(2,38)=1.90, P=0.163 2-Way ANOVA: F(1,6)=0.37, P=0.564
4A 2 5    2 5 Pass Fail Rank sum: T(7,7)=30.0, P=0.002  
4B 2 5    2 5 Fail   Rank sum: T(7,7)=28.0, P<0.001  
4C 2 5    2 5 Fail   Rank sum: T(7,7)=29.0, P=0.001  
4D 2 5    2 5 Fail   Rank sum: T(7,7)=28.0, P<0.001  
4E 2 5    2 5 Pass Pass Student’s t: t-test: t(12)=-4.01, P=0.002  
4F 5 5    5 5 Pass Pass 2-Way ANOVA: F(1,16)=55.42, P<0.001 2-Way ANOVA: F(1,16)=0.002, P=0.966
4G 5 5    5 5 Pass Pass 2-Way ANOVA: F(1,16)=55.42, P<0.001 2-Way ANOVA: F(1,16)=14.36, P=0.002
5A-nuc 7 2    6 2 Pass Fail Rank sum: T(8,9)=108.0, P<0.001  
5A-cyto 7 2    6 2 Fail   Rank sum: T(8,9)=108.0, P<0.001  
5A-memb 7 2    6 2 Fail   Rank sum: T(8,9)=105.0, P=0.002  
5B-nuc 7 2    6 2 Pass fail Rank sum: T(8,9)=107.0, P<0.001  
5B-cyto 7 2    6 2 Fail   Rank sum: T(8,9)=105.0, P=0.002  
5B-memb 6 2    6 2 Fail   Rank sum: T(8,8)=36.0, P<0.001  
  1. YM young males, YF young females, MM middle-aged males, MF middle-aged females, OM old males, OF old females, EV equal variance, FDR corrected for multiple comparisons using false detection rate
  2. aEqual variance only tested when dataset passed normality
  3. bEffect of sex only analyzed in experiments where n>4/sex/age. Rank sum used when 2-way ANOVA for age x sex failed normality or equal variance and reports effect of sex within old mice. 2-way ANOVA used when normality and equal variance pass and reports main effect of sex across ages
  4. cSee the “Results” section for post hoc tests