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Table 1 Primary and secondary antibodies for Western blot and immunofluorescence staining

From: EETs/sEHi alleviates nociception by blocking the crosslink between endoplasmic reticulum stress and neuroinflammation in a central poststroke pain model

Antibody

Provider

Host

Catalog number

Dilution for WB

Dilution for IF

BIP

Affinity

Rabbit

AF5366

1:1000

1:150

p-IRE1

Affinity

Rabbit

AF7150

1:500

1:150

p-PERK

Affinity

Rabbit

DF7576

1:500

1:150

ATF6

Affinity

Rabbit

DF6009

1:1000

1:150

XBP1

Proteintech

Rabbit

25997-1-AP

1:1000

 

p-eIF2a

Affinity

Rabbit

AF3087

1:500

 

eIF2a

Proteintech

Rabbit

11170-1-AP

1:1000

 

IRE

Proteintech

Rabbit

27528-1-AP

1:1000

 

PERK

Proteintech

Rabbit

20582-1-AP

1:1000

 

p-JNK

CST

Rabbit

#4671

1:500

 

JNK

CST

Rabbit

#9251

1:500

 

p-p38

CST

Rabbit

#9216

1:500

 

p38

CST

Rabbit

#14451

1:500

 

GAPDH

Proteintech

Mouse

60004-1-Ig

1:1000

 

Tublin

Proteintech

Mouse

66031-1-Ig

1:1000

 

NeuN

Abcam

Mouse

Ab104224

 

1:200

IBA1

Abcam

Goat

Ab5076

 

1:200

GFAP

Abcam

Mouse

MAB360

 

1:200

Anti-rabbit IgG HRP

Aspen

Goat

AS1107

1:4000

 

Anti-mouse IgG HRP

Aspen

Goat

AS1106

1:4000

 

Alexa Fluor 488-AffiniPure anti-mouse IgG

Jackson

Goat

115-545-003

 

1:500

Alexa Fluor 594-AffiniPure anti-rabbit IgG

Jackson

Goat

111-585-003

 

1:500

Alexa Fluor 488-AffiniPure anti-goat IgG

Jackson

Donkey

705-545-003

 

1:500

Alexa Fluor 594-AffiniPure anti-rabbit

Jackson

Donkey

711-585-152

 

1:500

  1. Abbreviations: BIP, binding immunoglobulin protein; IRE1, inositol-requiring kinase 1; PERK, protein kinase RNA-like endoplasmic reticulum kinase; ATF6, activating transcription factor 6; XBP1, X-box-binding protein 1; eIF2α, eukaryotic initiation factor 2α; JNK, c-jun NH2-terminal kinase; IBA1, ionized calcium-binding adapter molecule 1; NeuN, neuronal nuclei; GFAP, glial fibrillary acidic protein; HRP, horseradish peroxidase