High-fat diet increases gliosis and immediate early gene expression in APOE3 mice, but not APOE4 mice

Table 1 Primer sequences for qRT-PCR

Primer	Sequence	Temperature
Adora2a	Fwd: AGCAACCTGCAGAACGTCACAAAC Rev: TGGCAATAGCCAAGAGGCTGAAGA	60
Arc	Fwd: GGAGGGAGGTCTTCTACCGTC Rev: CCCCCACACCTACAGAGACA	57.5
C3	Fwd: GACGCCACTATGTCCATCCT Rev: CCAGCAGTTCCAGGTCCTTTG	57.5
cFos	Fwd: CTCTGGGAAGCCAAGGTC Rev: CGAAGGGAACGGAATAAG	55
Egr1	Fwd: GAGGAGTTATCCCAGCCAA Rev: GGCAGAGGAAGACGATGAAG	57.5
GAPDH	Fwd: GTGTTTCCTCGTCCCGTAGA Rev: ATTCCGTTCACACCGACCTT	55, 57.5, 60
IL3	Fwd: CCTGGGACTCCAAGCTTCAA Rev: GACAATAGAGCTGCAATTCAACGT	57.5
Il6	Fwd: ACGGCCTTCCCTACTTCACA Rev: CATTTCCACGATTTCCCAGA	57.5
TNF-a	Fwd: GGTGCCTATGTCTCAGCCTCTT Rev: GCCATAGAACTGATGAGAGGGAG	60

ISSN: 1742-2094