Fig. 1
From: 1α,25-dihydroxyvitamin D3 protects retinal ganglion cells in glaucomatous mice
Pharmacological treatment. A Experimental design. Baseline PERG and FERG recordings were performed before treatment. The 1,25(OH)2D3 treatment was carried out, for 5 days per week, for 5 weeks. PERG/FERG recordings were carried out once per week. At the end of 5 weeks, mice were sacrificed, and tissues processed for immunohistochemical staining and molecular biology analyses. B Immunohistochemistry images sampling on retinal on whole mount. For each retina, 8 images were acquired, 4 related to central retina and 4 from peripheral retina. Six whole mounts per experimental group were analysed. A total of 48 images per experimental group provided RBPMS+, Iba-1+, and GFAP+ cells quantifications. Both periphery and central images were acquired at 500-μm distance, from whole mount edge and optic disc hole, respectively