Fig. 4
From: 1α,25-dihydroxyvitamin D3 protects retinal ganglion cells in glaucomatous mice
RGCs staining in flat-mount retinas. Upper panel shows representative images of Rbpms+ RGCs in whole-mount retinas of control (vehicle-treated) and 1,25(OH)2D3-treated mice. Scale bar corresponds to 50 μm. RGCs density was significantly higher in 1,25(OH)2D3-treated mice, compared to the control group. Quantification was carried out on whole retina (A) and also on both central and peripheral areas of retina (B). Data were plotted as mean ± SD (n = 6 flat-mount retinas for each experimental group, n = 48 images per experimental group). A *p = 0.036 vs. Ctrl; t-test two-tailed. B *p = 0.0011 vs. Ctrl periphery (Ctrl p) ‡p = 0.0011 vs. Ctrl centre (Ctrl c), one-way ANOVA with Tukey post hoc test for multiple comparison