Fig. 5
From: 1α,25-dihydroxyvitamin D3 protects retinal ganglion cells in glaucomatous mice
The 1,25(OH)2D3 and retinal neurotrophic factors. The 1,25(OH)2D3 increased retinal expression of neuroprotective factors in DBA/2J mice: BDNF (A), VEGF-A (B), and PlGF (C) mRNA expression. Retinal samples of 1,25(OH)2D3-treated and control mice were collected at the end of treatment. Values represent the mRNA fold changes relative to 18S, a housekeeping gene. Each bar represents the mean value ± SD (n = 6 for each experimental group, two pooled retinas per independent sample, each run in triplicate). The 1,25(OH)2D3 vs. control p = 0.0006 for BDNF, p = 0.0069 for VEGF-A, p = 0.0088 for PlGF (unpaired and two-tailed t-test)