Fig. 8
From: 1α,25-dihydroxyvitamin D3 protects retinal ganglion cells in glaucomatous mice
Effect of 1,25(OH)2D3 on activation of NF-κB and expression of cytokines in DBA/2J mice. A Representative blots of phospho-NF-κB-p65, total NF-κB-p65, and GAPDH in DBA/2J retinas (n = 6 independent samples for each group, 2 pooled retinas per sample). Expression of phospho-NF-κB-p65 and total NF-κB was quantified using densitometric analysis B. Each bar represents the mean value ± SD (n= 6). *p = 0.0040 vs. control. The 1,25(OH)2D3 reduced IL-6 (*p = 0.0117, C), IL-1β (*p = 0.0412, D), IFN-γ (*p = 0.0266, E), and CCL-3 (*p = 0.0100, F) mRNA expression, compared to control. The mRNA levels were evaluated by qPCR; values represent the mRNA fold changes relative to 18S used as resident control. Each bar represents the mean value ± SD (n = 6 independent samples for each group, 2 pooled retinas per sample, each run in triplicate),(unpaired and two-tailed t-test)