Fig. 2

The microenvironment of parent hippocampal sclerotic tissue is anti-neurogenic. A An intraoperative photograph of the human hippocampus at the end of a trans-sylvian dissection from a patient with mTLE. B Bright-phase snapshot (5× field) of 3D hippocampal slice culture at 21DIV. C A schematic outline of the pulse and chase protocol used to quantify neurogenesis in 3D cultures using a BrdU or EdU and NeuN or TuJ1, respectively. D–G Quantification of neurogenesis (TuJ1⁺cells) in cells dissociated from secondary neurospheres generated from the same patient. H The neurogenic capacity of cells grown in ideal microenvironment was ten times higher than those grown in 3D hippocampal cultures generated from the same patient Values are means ± SE based on samples generated from 3 different patients (scale bar for neurosphere cultures is 100 μm and for monolayer adherent cultures is 10 μm)