Fig. 3

Tregs depletion aggravated NMOSD lesion development, while adoptive transfer of Tregs inhibited lesion progression. A Representative flow cytometric identification of anti-CD25mAb antibodies used to deplete CD4⁺CD25⁺Foxp3⁺ Tregs gated on CD4⁺ T cells in spleen at 9 days after injection. B Quantitative analysis of CD4⁺CD25⁺Foxp3⁺ Tregs out of CD4⁺ T cells. n = 5–6 per group. **p < 0.01 versus PBS group; statistical analyses were performed by Mann-Whitney U test. C Representative histological recognition of adoptively transferred Fxop3-EGFP⁺ Tregs in NMOSD lesions. Scale bar, 20 μm. D Quantification of the number of adoptively transferred Foxp3-EGFP⁺ cells. n = 5 per group. **p < 0.01 versus PBS group; statistical analyses were performed by Mann-Whitney U test. E Representative images of the white matter lesions by LFB staining. Scale bar, 300 μm. F Quantitative analysis of lesion size. n = 5 per group. **p < 0.01, ***p < 0.001, versus NMOSD group; statistical analyses were performed by one-way analysis of variance followed by Dunnett’s post hoc test. G Representative images of AQP4, GFAP, and MBP staining area in different groups. Scale bar, 80 μm. H Quantification of AQP4, GFAP, and MBP area of staining. n = 5 mice for each group. *p<0.05, **p < 0.01, ***p < 0.001, versus NMOSD group; statistical analyses were performed by one-way analysis of variance followed by Dunnett’s post hoc test. I Representative TEM images of myelination in the corpus callosum of mice. Scale bar, 1 μm