Fig. 4

NMOSD-IgG and hC induced inflammatory activation and infiltration were partially enhanced by Tregs ablation but reversed by adoptive transfer of Tregs. A Representative images of CD45 and Iba1 staining. Scale bar, 300 μm. B Quantitative analysis of CD45 and Iba1 staining areas. n = 5 per group. *p < 0.05, **p < 0.01. ns, no significance, versus NMOSD group; statistical analyses were performed by one-way analysis of variance followed by Dunnett’s post hoc test. C Representative images of Ly-6G and CD3 staining. Scale bar, 300 μm. D Quantitative analysis of Ly-6G⁺ and CD3⁺ cell numbers. n = 5 per group. *p < 0.05, **p < 0.01. ns, no significance, versus NMOSD group; statistical analyses were performed by one-way analysis of variance followed by Dunnett’s post hoc test