Fig. 3

Interfering with HOXA-AS2 suppresses LPS-induced M1 microglial polarization, promotes polarization to the M2 phenotype, and ameliorates impaired behaviors in LPS-injected mice. A Effects of intraventricular injection of HOXA-AS2 siRNA on HOXA-AS2 mRNA expression. B Effects of intraventricular injection of HOXA-AS2 siRNA on M1 and M2 markers in the substantia nigra as determined by RT-qPCR in LPS-injected mice. C Effects of intraventricular injection of HOXA-AS2 siRNA on LPS-induced impaired behavior as determined by open-field tests where the number of times the mice stood on their hind legs (rearings) and the number of squares crossed by the mice were recorded. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus control group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus the LPS-injected only group (n = 8/group, one-way ANOVA). Data are represented as the mean ± SD