Fig. 4

HOXA-AS2 suppresses PGC-1α transcription to regulate microglial polarization. A Representative Western blotting showing PGC-1α protein levels in BV2 cells transfected with HOXA-AS2 plasmid or HOXA-AS2i. (^*P < 0.05, ^**P < 0.01 versus control group, Student’s t test). B RT-qPCR analysis of M1 markers in BV2 cells transfected with HOXA-AS2 and PGC-1α plasmids. C RT-qPCR analysis of M2 markers in BV2 cells transfected with HOXA-AS2i and PGC-1αi. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus HOXA-AS2oe or HOXA-AS2i groups (one-way ANOVA). The data are from three independent experiments and represented as mean ± SD