Fig. 5

HOXA-AS2 acts as a modular scaffold of PRC2 in the nucleus. A Relative HOXA-AS2 expression levels in nuclear and cytosolic fractions. Nuclear controls: NEAT1; cytosolic controls: GAPDH. B RIP experiments for EZH2, SUZ12, RbAp48, and RING1A/B were performed, and the coprecipitated RNAs were subjected to RT-qPCR for HOXA-AS2. GAPDH was employed as a negative control. C RNA pulldown was used to examine the direct interaction between HOXA-AS2 and EZH2 in BV2 cells. The EZH2 protein levels were evaluated by Western blot. AR (Antisense RNA) was used as a positive control. ^***P < 0.001 versus control group (Student’s t test). The data are from three independent experiments and represented as mean ± SD