Fig. 6

HOXA-AS2 participates in the epigenetic repression of PGC-1α by interacting with PRC2. A Representative Western blotting showing PGC-1α and EZH2 protein levels in BV2 cells transfected with EZH2i. B RT-qPCR analysis of M1 and M2 markers in BV2 cells transfected with EZH2i. C ChIP assays were performed to detect EZH2 and H3K27me3 occupancy in the PGC-1α promoter region. Enrichment of DNA was quantified relative to the amount of input by RT-qPCR. IgG was used as a negative control. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus control group (Student’s t test). The data are from three independent experiments and represented as mean ± SD