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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury

Fig. 1

After traumatic SCI, astrocytes release more sEVs that encapsulate CCL2. a, b Western blotting to detect the expression of CCL2 in spinal cord tissue. c qRT-PCR to detect the expression of CCL2 in spinal cord tissue. d, e Immunofluorescence co-localization of astrocyte markers GFAP and CCL2. f TEM observation of sEVs released by cells in spinal cord tissue. g TEM observation of the typical structure of sEVs purified from spinal cord tissue. h NTA analysis of sham-sEVs and SCI-sEVs, showing that the small extracellular vesicles in the two groups had similar size ranges. i, j Western blotting analysis of sEV marker proteins TSG101, CD63, CD9 and CD81. k BCA method to determine the protein concentrations of the two groups of sEVs. l, m Western blotting to detect the expression of CCL2 in sEVs

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Journal of Neuroinflammation

ISSN: 1742-2094