Fig. 3

sEVs released by activated astrocytes induce microglial activation and neuronal apoptosis. a–c ELISA method to detect the expression of TNF-α, IL-1β, and IL-6. d Detection of changes in NO content. e, f Transwell assessment of the migration ability of BV2 microglia. g, h TUNEL staining detecting neuronal apoptosis. i, j Western blotting detecting the expression of apoptosis-related proteins in neurons